Environmental Transition of Signal-Anchor Sequences during Membrane Insertion via the Endoplasmic Reticulum Translocon

摘要

We determined the environments of polypeptide chains during membrane translocation and integration using site-directed Cys alkylation. Migration of a signal-anchor sequence into the membrane synchronizes with formation of its TM orientation, and the ER translocon can provide the aqueous pathway capable of two hydrophilic chains.